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Generation of stable co-cultures of vascular cells in a honeycomb alginate scaffold.

TitleGeneration of stable co-cultures of vascular cells in a honeycomb alginate scaffold.
Publication TypeJournal Article
Year of Publication2010
AuthorsYamamoto M, James D, Li H, Butler J, Rafii S, Rabbany S
JournalTissue Eng Part A
Volume16
Issue1
Pagination299-308
Date Published2010 Jan
ISSN1937-335X
KeywordsAlginates, Cells, Cultured, Coculture Techniques, Endothelial Cells, Glucuronic Acid, Hexuronic Acids, Humans, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, Neovascularization, Physiologic, Porosity, Tissue Engineering
Abstract

Scaffold-guided vascular tissue engineering has been investigated as a means to generate functional and transplantable vascular tissue grafts that increase the efficacy of cell-based therapeutic strategies in regenerative medicine. In this study, we employed confocal microscopy and three-dimensional reconstruction to assess the engraftment and growth potential of vascular cells within an alginate scaffold with aligned pores. We fabricated honeycomb alginate scaffolds with aligned pores, whose surface was immobilized with fibronectin and subsequently coated with matrigel. Endothelial cells were seeded into aligned pore scaffolds in the presence and absence of human smooth muscle cells. We showed that endothelial cells seeded into alginate scaffolds attach on the surface of aligned pores in vitro, giving rise to stable co-cultures of vascular cells. Moreover, the three-dimensional alginate depots containing the cells were exposed to laminar flow in order to recapitulate physiological shear stress found in the vasculature in vivo. After the flow exposure, the scaffold remained intact and some cells remained adherent to the scaffold and aligned in the flow direction. These studies demonstrate that alginate scaffolds provide a suitable matrix for establishing durable angiogenic modules that may ultimately enhance organ revascularization.

DOI10.1089/ten.TEA.2009.0010
Alternate JournalTissue Eng Part A
PubMed ID19705957
PubMed Central IDPMC3120091
Grant ListP01 HL059312 / HL / NHLBI NIH HHS / United States
P01 HL059312-060006 / HL / NHLBI NIH HHS / United States
P01 HL059312-070006 / HL / NHLBI NIH HHS / United States
P01 HL059312-080006 / HL / NHLBI NIH HHS / United States
P01 HL059312-090006 / HL / NHLBI NIH HHS / United States
P01 HL059312-100006 / HL / NHLBI NIH HHS / United States
P01 HL067839 / HL / NHLBI NIH HHS / United States
P01 HL067839-010004 / HL / NHLBI NIH HHS / United States
P01 HL067839-020004 / HL / NHLBI NIH HHS / United States
P01 HL067839-030004 / HL / NHLBI NIH HHS / United States
P01 HL067839-040004 / HL / NHLBI NIH HHS / United States
P01 HL067839-050004 / HL / NHLBI NIH HHS / United States
P50 HL084936 / HL / NHLBI NIH HHS / United States
P50 HL084936-010003 / HL / NHLBI NIH HHS / United States
P50 HL084936-020003 / HL / NHLBI NIH HHS / United States
P50 HL084936-030003 / HL / NHLBI NIH HHS / United States
P50 HL084936-040003 / HL / NHLBI NIH HHS / United States
R01 HL058707 / HL / NHLBI NIH HHS / United States
R01 HL058707-03 / HL / NHLBI NIH HHS / United States
R01 HL058707-04 / HL / NHLBI NIH HHS / United States
R01 HL061849 / HL / NHLBI NIH HHS / United States
R01 HL061849-02 / HL / NHLBI NIH HHS / United States
R01 HL061849-03 / HL / NHLBI NIH HHS / United States
R01 HL061849-03S1 / HL / NHLBI NIH HHS / United States
R01 HL061849-04 / HL / NHLBI NIH HHS / United States
R01 HL061849-05 / HL / NHLBI NIH HHS / United States
R01 HL075234 / HL / NHLBI NIH HHS / United States
R01 HL075234-01 / HL / NHLBI NIH HHS / United States
R01 HL075234-02 / HL / NHLBI NIH HHS / United States
R01 HL075234-03 / HL / NHLBI NIH HHS / United States
R01 HL075234-04 / HL / NHLBI NIH HHS / United States
R01 HL097797 / HL / NHLBI NIH HHS / United States
R01 HL097797-01 / HL / NHLBI NIH HHS / United States
R01 HL097797-02 / HL / NHLBI NIH HHS / United States
R01 HL097797-03 / HL / NHLBI NIH HHS / United States
R21 HL083222 / HL / NHLBI NIH HHS / United States
R21 HL083222-01 / HL / NHLBI NIH HHS / United States
R21 HL083222-02 / HL / NHLBI NIH HHS / United States
RC1 AI080309 / AI / NIAID NIH HHS / United States
RC1 AI080309-01 / AI / NIAID NIH HHS / United States
U01 HL066952 / HL / NHLBI NIH HHS / United States
U01 HL066952-010002 / HL / NHLBI NIH HHS / United States
U01 HL066952-020002 / HL / NHLBI NIH HHS / United States
U01 HL066952-030002 / HL / NHLBI NIH HHS / United States
U01 HL066952-040002 / HL / NHLBI NIH HHS / United States
U01 HL066952-050002 / HL / NHLBI NIH HHS / United States